Virtual Diegest (ISPaR) · T-RFLP Analysis (APLAUS+) · T-RFLP Analysis (PAT+) · P.S.P.A. · E.R.P.A.
June 18, 2013
  MiCA: T-RFLP Analysis (PAT+)
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Restriction sites are identified within the gene fragment delimited by the primers. The number of base pairs between the 5’ and 3’ primers and the proximal restriction sites are reported.
     
Forward Primer:
  
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    (CSV or TXT file ONLY!)
Reverse Primer:
  
or enter here:
  
Restriction Enzyme:
  
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Select Database:
  
Sensitivity:
   digest allows at most     mismatches within     bases from 5' end of primer
   
Window (Bin) Size:
   forward match: ±     bps.
Sort By:
   Forward fragments Abundance Name
Sort Order:
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If you use the data generated by MiCA in a publication, please cite:
Shyu, C., Soule, T., Bent, S.J., Foster, J.A., Forney, L.J. (2007) MiCA: A Web-Based Tool for the Analysis of Microbial Communities Based on Terminal-Restriction Fragment Length Polymorphisms of 16S and 18S rRNA Genes. Journal of Microbial Ecology 53:562-570.
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Last updated on April 08 2011 00:23:26.